rna probes การใช้

The photoactivatable group provides nonspecific labeling of proteins, DNA and RNA probes or other molecules.
RNA probes complementary to mRNA targets trigger chain reactions in which fluorophore-labeled scRNAs self-assemble into tethered fluorescent amplification polymers.
By using RNA probes, Todd Oakley research group determined that one of the two cryptochromes, Aq-Cry2, was produced near the sponge s simple eye cells.
The probe hybridizes to the target sequence at elevated temperature, and then the excess probe is washed away ( after prior hydrolysis using RNase in the case of unhybridized, excess RNA probe ).
The recombinant DNA may also be detected using other methods such as nucleic acid hybridization with radioactive RNA probe, while cells that expressed the desired protein from the plasmid may also be detected using immunological methods.